CHEN 3701 Lecture Notes - Lecture 31: Tetrapeptide, Signified And Signifier, Bundesautobahn 61
8 Nov 2015
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12:24 pm: you created a combinatorial library of ligands in which ten codons within a peptide loop were diversified using nnn degenerate codons (equal probability of each nucleotide at all three codon positions). After testing ten thousand proteins from the library, you found a high affinity ligand with a serine-histidine-proline-glutamine (shpq) tetrapeptide motif. Write them in the context (single- or double- stranded) that they most frequently appear in the cell. Dna & rna sequences one solution: mrna (single-stranded) will match the codons on the table, not complement them only u"s are changed to ts. 5" - ucucauccucaa - 3" corresponding dna: (double stranded) 3"-agagtaggagtt-5" bottom = template strand: what is the likelihood that the library contained a functional ligand with the shpq motif? consider the probability of each amino acid being encoded by an nnn codon at a given site p(p)=4/64. -> from codon chart; ex: 6 codons out of 64 that encode for s.
