CHEN 3701 Lecture Notes - Lecture 32: Selectable Marker, Telomerase Reverse Transcriptase, List Of Bluetooth Profiles
8 Nov 2015
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Material up to this point is on exam (focus on material after exam 1, but builds on exam 1 material) Aim: produce functinal proteins efficiently and with high quality. Functional proteins - catalysis binding (purification, diagonstics, pharamceutical) structural. In 2014, 7 of the tip 12 selling pharmaceuticals were proteins (biologics) 2 humira . 16b tnf antibody 150 kda cho (chinese hamster ovary) 5 enbrel . 2b tnfr-fc fusion 150 kda cho. 8 remicade . 7b tnf antibody 150 kda murine myeloma. 9 lantus . 7b insulin 6 kda e. coli bacteria. 10 neulasta . 4b pegylated gcsf 19 kda + 20 kda e. coli. 11 copaxone . 4b tetrapeptide 1 kda chemical synthesis. 12 rituxan . 7b cd20 antibody 150 kda cho. Cho is common way to produce complex large proteins. 5 grabs the natural moleule and fuses to fc part of antibody. Tnfr - fc is for binding to tnfalpha fc is pk murine myeloma = mouse myeloma. Not efficient for most proteins (medium or large size)
