CHEN 3701 Lecture Notes - Lecture 40: Inta, Selectable Marker, Reaction Rate Constant
22 Nov 2015
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Dna (plasmid) cell put dna with our desired gene in the cell, make the cells make the product usually do this in batch reactor segregational loss. Daughter loss: usually, we produce 2 copies of cells with plasmid dna and their own set of chromosomesbut now the 2nd cell (daughter cell) lacks plasmidso it replicates and uses up substrate, but doesn"t give you your roduct. Low copy: 1-2 plasmids per cell plasmid instability (don"t lose it, but its integrity is bad) Mutation that enables growth but lacks ability to produce functional protein host cell mutation. Genomic mutation these changes can impact cell growth. Daughter loss is bad since daughter cells w/o plasmid replicate faster since they don"t make product. Selectable marker can slow growth of plasmidless cells. But in lack of selectivity, plasmidless cells may grow faster due to lack fo resource use on [p] formation. Host cell mutations tend to slow growth of all cells.
