HMED 3075 Lecture Notes - Lecture 8: Victorian America, Hemocytometer, Microscope Slide
Document Summary
Lecture: gender, disease, and sexuality in victorian america. Reading: keith wailoo, (cid:862)(cid:858)chlorosis(cid:859) e(cid:373)e(cid:373)(cid:271)ered: disease a(cid:374)d the moral ma(cid:374)age(cid:373)e(cid:374)t of a(cid:373)eri(cid:272)a(cid:374) wo(cid:373)e(cid:374). (cid:863) Technologies of blood analysis c. late 19th century. Collecting blood for analysis: locate blood vessel, puncture, and withdraw blood, prick a finger or earlobe. Smear blood on microscope slide: not too tick/thin, no contamination, skilled work. Hemocytometer: counting grid part of microscope slide. Count cells in a grid rather than entire slide. Attempt to correlate blood counts with clinical disease. Method of estimating amount of hemoglobin in the blood. Compared the color of blood with the color of a known standard. Standard tube: glycerine jelly colored to correspond to 1% solution of normal blood. Second tube with graduated scale: fixed amount of sample blood; water slowly added to dilute blood to the color of the standard. If blood has high levels of hb, a lot of water needs to be added. Spin sample in small tube using centrifuge.
