BIOL 205 Lecture Notes - Lecture 3: Cell Membrane, Sonication, Detergent
Document Summary
Homogenate thick soup of cell extract produced when cells are broken apart. Learning objective #1 describe different strategies to break open cells and isolate cellular organelles. Centrifugation technique that separates a homogenate into different parts: differential centrifugation repeated centrifugation at different speeds separates cell components based on size and density. Chromatography a matrix of small beads that separates proteins according to charge, size, hydrophobicity, or ability to bind to a specific molecule. They are released by a solvent that alters the ph of the column: gel-filtration chromatography small molecules that enter holes in the beads are what bind to the column. They are released by a solvent that washes away the molecules or by time/ gravity: affinity chromatography proteins that interact with the substrate molecule on the beads are what bind to the column. They are released by a solvent that alters the ph of the column: gives a very high purity.
