CHEM 2410 Lecture Notes - Lecture 1: Telomerase Reverse Transcriptase, Chromatography, Acetanilide
12 Sep 2016
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Terpenes are composed from 5 carbon isoprene units, produced by plants and animals. The different isomers should have identical physical properties, but when they react with enzymes, your nose can detect different forms. Ex: monoterpene-dervied from 2 isoprene units, sesquiterpene c15- 3 isoprene, diterpene-c20- 4 isoprene. Co2-prevents waste, avoid accident prone materials, co2 isnt toxic. Principle of separation-terpenes are non polar organics. We break up matrix of peal, mix with liquid co2, filter off insoluble material, Co2 evaporates off: at elevated pressures, co2 exists as liquid and gas, super critical fluid. One partner does lemon peel, one does orange peel, then each person has to write up individual reports for both samples. Put in centrifuge tube equipped with filtration flask (w/ copper coil) Converts to liquid, and it drains through filter, and liquid evaporates in 3-4 minutes. When co2 is gone, open tube behind hood. Take out solid orange peel and weigh flask.
