MATH 003 Lecture Notes - Lecture 18: Enzyme Kinetics, Reaction Rate, Enzyme
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Enzyme kinetics is the study of the chemical reactions that are catalysed by enzymes. In enzyme kinetics, the reaction rate is measured and the effects of varying the conditions of the reaction are investigated. Studying an enzyme"s kinetics in this way can reveal the catalytic mechanism of this enzyme, its role in metabolism, how its activity is controlled, and how a drug or an agonist might inhibit the enzyme. Enzymes are usually protein molecules that manipulate other molecules the enzymes" substrates. These target molecules bind to an enzyme"s active site and are transformed into products through a series of steps known as the enzymatic mechanism. E + s es es* ep e + p. These mechanisms can be divided into single-substrate and multiplesubstrate mechanisms. E + s es * ep e + p. Enzymes with single-substrate mechanisms include isomerases such as triosephosphateisomerase or bisphosphoglycerate mutase, intramolecular lyases such as adenylate cyclase and the hammerhead ribozyme, an rna lyase.