BSCI-1510 Chapter Notes -Okazaki Fragments, Dna Replication, Semiconservative Replication
Document Summary
Chapter fourteen: understand the basic structure of double-stranded dna: base pairing, double helical, sugar phosphate backbone, anti-parallel pairing. No need to recall any of the historical experiments which led to understanding dna structure nor the 3 forms (a, B, and z) of dna structure: main features of dna double-helix: i. ii. Dna consists of 2 strands hydrogen-bonded together with a width = 2 nm. The 2 strands turn right (clockwise) to make a right-handed helix: strands are like the ropes of a ladder with the bases as the rungs iii. The hydrophilic sugar-phosphate is on the outside of the helix: the negative phosphate groups make dna soluble in aqueous iv. v. vi. vii. viii. ix. solution. The hydrophobic nitrogenous bases are stacked in a perpendicular manner to the strand: distance between 2 base pairs = 0. 34 nm, 10 base pairs per turn.
