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23 Jun 2019

I need help with the discussion and conclusion. This is the correct result from the experiment and correct format for the lab report.

Objective

Antibiotic disc test (Perform antibiotics test assay)

Introduction

The purpose of this experiment is to learn how to test the susceptibility of microorganisms to various antibiotics. Antibiotic is a chemical produced by a fungus or bacterium that has the potential to limit the growth of another bacterium.

Material and Methods

The description is in the laboratory manual. Test 1 using the aseptic technique to paint the entire surface of a sterile agar plate with E. coli and to covers all the areas then place four different antibiotics disc onto the marked surface with their proper name onto it.

Results

Table 1: Antibiotic inhibition zone results for E. coli

Diameter – Disc/2 = Radius (r)

Disc = 6mm/10 = 0.6 cm.

Bacterium: E. coli

Antibiotic used

Inhibition zone size (cm)

Penicillin 10 mg/1

0

Ampicillin

3.8 – 0.6/2 = 1.6

Penicillin 100 mg/1

1.70 – 0.6/2 = 0.55

Cefoxitin

4.20 – 0.6/2 = 1.8

Discussion/conclusion

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Lelia Lubowitz
Lelia LubowitzLv2
25 Jun 2019

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