In biochemistry lab (DNA Isolation by column, and precipiation method, Agarose gel of isolated DNA, and Protein Colorimetric assay) Questions
Three recommendations for the future: In the âreal worldâ, researchers generally have many chances to practice and refine their techniques and procedures.If you had to repeat this experiment, what three things would you focus on doing differently?
Focus on things that can be achieved in the time that we have available and using equipment that we already have.
You may have other ideas, but consider these options:
* Do you think that there was a way to break open the strawberry cells more efficiently?
* Do you think that your DNA was at a suitable concentration to allow you to work with it effectively â or should it perhaps have been resuspended/eluted in a smaller/larger volume?
* Do you think that you had enough replicates of the DNA concentration in order for you to know whether you were getting an accurate reading?
* Did you need more practice pipetting small volumes before moving on to using the NanoDrop? Did you need more practice using the NanoDrop itself?
* Do you think that there is a step that you could have performed more quickly or with less error (e.g. where you lost time or spilled sample, but could improve on this next time)?
In biochemistry lab (DNA Isolation by column, and precipiation method, Agarose gel of isolated DNA, and Protein Colorimetric assay) Questions
Three recommendations for the future: In the âreal worldâ, researchers generally have many chances to practice and refine their techniques and procedures.If you had to repeat this experiment, what three things would you focus on doing differently?
Focus on things that can be achieved in the time that we have available and using equipment that we already have.
You may have other ideas, but consider these options:
* Do you think that there was a way to break open the strawberry cells more efficiently?
* Do you think that your DNA was at a suitable concentration to allow you to work with it effectively â or should it perhaps have been resuspended/eluted in a smaller/larger volume?
* Do you think that you had enough replicates of the DNA concentration in order for you to know whether you were getting an accurate reading?
* Did you need more practice pipetting small volumes before moving on to using the NanoDrop? Did you need more practice using the NanoDrop itself?
* Do you think that there is a step that you could have performed more quickly or with less error (e.g. where you lost time or spilled sample, but could improve on this next time)?
