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17 Jun 2018

You need to make 550ml of a 6.5% (w/v) sodium nitrite (NaNO2) solution as a food preservative to prevent microbial growth. How many grams of NaNO2 (f.w.=68.995 g/mol) will you need? What is the molarity (M)of this solution?

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Bunny Greenfelder
Bunny GreenfelderLv2
19 Jun 2018

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Related questions

3. You have a 5 M NaCl stock solution. (NaCl F.W. is 58.4)

a) How much NaCl (in g) do you need to make 1 liter of the 5 M stock solution?

b) How much stock solution will you need if you want to make 100 ml of 50 mM NaCl?

c) What is the % concentration of a 50 mM solution of NaCl?

BACTERIAL GROWTHand CONTROLLINGBACTERIAL GROWTH lab report using virtual unknownsoftware

Experiment II:Controlling Microbial GrowthIntroduction

<Include purpose of lab experiment>

<brief summary of topic investigating and case studies>

<state major finding>

Procedure

< Include information that the reader would need to repeatyour experimental procedure. Do not include any observations orresults in this section>

Observations andResults

<Gram Stain Result>

Discussion &Conclusion

Include the answers to the following questions:

  • Salt is one of the oldest food preservatives. How does saltcontrol microbial growth and reduce spoilage? Which two mediainclude elevated concentrations of NaCl? Would bacteria that havethe ability to grow in these two media be more likely to cause foodspoilage? Why or why not?

  • Bile salts are natural products of the digestive system thatprevent growth of many bacteria. How do bile salts exertantimicrobial activity? What is the concentration of bile salts inbile esculin agar? Why might some bacteria be able to grow on thismedium?

  • Nitrate is often used as a preservative in cured meats toprevent anaerobic growth of spoilage bacteria. Why would theinclusion of nitrate inhibit anaerobic growth?

  • Potassium cyanide (KCN) is a potent poison. How might somebacteria be able to grow on media containing potassium cyanide?

    Conclusion

    < Within a few sentences, provide a concluding statementabout the results of both your laboratory>

10. When you make your agarose gel, you will need to make 50 ml of 1% (w/v) agarose in 1X TAE. How much agarose will you require? [you will make this later, not on Solution day] When adding your DNA samples to the agarose gel, you will need to add loading dye to the samples. Loading dye contains: • glycerol, which helps the samples sink into the sample wells of the gel; and • tracking dyes so that you have a way of estimating how far the samples have travelled through the gel during electrophoresis. The recipe for 6X loading dye is as follows: • 0.25% bromophenol blue (w/v) • 0.25% xylene cyanol (w/v) • 30% glycerol in water (v/v)

11. Provide a recipe for 10 ml of 10X loading dye, using the information above. (Important tip: Remember that you have already made 30ml of a 50% stock solution of glycerol. I recommend determining the concentration of glycerol required for 10X loading dye, then figuring out how much of your 50% glycerol stock will be needed to make 10ml of 10X loading dye.)

Solution to make

Exact pH of final solution (if appropriate)

Final container for solution

Completed (Y or N)

100 ml of 0.9% (w/v) NaCl

100 ml of 1.0M Tris, pH 7.6

40 ml of 50X TAE

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