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30 Mar 2019

Prokaryotes don't have a nucleolus but still produce a lot ofribosomal RNAs. Transcription of prokaryotic rRNA mostly likelyinvolved which of the following transcription factors? WHY?

a) Sigma 28

b) Sigma 38

c) Sigma 70

d) TBP (TATA Binding Protein)

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Bunny Greenfelder
Bunny GreenfelderLv2
31 Mar 2019

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Related questions

1. Bacterial transcription termination VS. Eukaryotic transcription initiation

a. What is required for Rho-independent termination? How does each sequence feature contribute to the mechanism of termination?

b. What is required for Rho-dependent termination?

c. How does Rho differ from other helicases we discussed in Chapter 28? How is it similar?

d. Do you understand the difference between core promoter elements and regulatory elements? Do you understand the difference between core transcription factors and regulatory transcription factors?

e. What are the major features of the TATA-binding protein-DNA interaction? How does this interaction lead to dsDNA destabilization?

1)How many fragments of DNA will be produced, if you digest pBR322with the restriction enzyme EcoR1?

2) (more than one answer) A mature eukaryotic mRNA containsthe following:
a)Introns
b)Exons
c)Poly A tail
d)A methylated guanine cap
e)UTR our side start and stop codons

3) Which of the following statements are correct ?
a)An eukaryotic promoter contains TATA box
b)A protein molecule terminates with one acidic and one basicends
c) Several transcription factors are involved in initiating agene transcription
d) An RNA primer is needed for gene transcription
e) RNA polymerase || is responsible for eukaryotic genetranscription

1 DNA sequences found in introns provide [A] amino acid sequence information.

[B] regulatory information.
[C] no known useful information.

[D] structure for the gene.
[E] alternative DNA splicing possibilities.

3. The TATA box is a(n)
[A] sequence close to the promoter region of many genes.
[B] square-shaped sequence.
[C] enhancer consensus sequence.
[D] activator sequence necessary for proper translation.
[E] None of the above

5. Regarding genes and gene expression, which of the following is not a difference between prokaryotes and eukaryotes
[A] Eukaryotic DNA does not contain a TATA box close to the initiation site as does prokaryotic DNA.

[B] In eukaryotes, RNA polymerase requires

'transcription factors'or initiation.

[C] Prokaryotic genes are often grouped together in

operons.

[D] In eukaryotes, the regulation of several genes at once requires common control elements in each of the genes.
[E] There is a diversity of eukaryotic polymerases that is reflected in the diversity of eukaryotic promoters.

10) In eukaryotic cells, a repressor

[A] is made of DNA.
[B] binds to the enhancer region to block transcription. [

C] is located both upstream and downstream from the promoter.
[D] binds to the operator to block RNA polymerase.

[E] binds to a silencer to reduce transcription rates.

Coordinated regulation of genes in eukaryotic cells [A] is the result of positioning the same regulatory sequence in front of each gene.
[B] results from all of those genes being under the control of one promoter.

[C] occurs because related genes all have the same

operons.
[D] occurs because enhancers cause DNA to bend.

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