1. How is the sedimentation rate used clinically ?

2. If you forgot to read the ESR results at the 60 minutes mark and discovered your mistake at 95 minutes mark, what action do you take and how do you interpret the results?

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Tod ThielLv2

8 Apr 2018

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QUESTIONS TO ANSWER:
1.Looking at Janeâs lab profile, it is indicative of what condition?
2.What are the two most common causes of this condition?
3.Which lab result is critical in the diagnosis of this disease?
4.Discuss the significance of the Hematology results?

Jane L) a 30-year-old woman, was seen in the ER with severe epigastric pain radiating to the back that woke her from sleep. She also complained of nausea and vomiting. The chemistry and hematology tests shown in Tables 2â8 and 2â9 were ordered.

Jane L. Reference Range

		Sodi um		140		135-145 mEq/L
		Potassium		3.6		3.6â5.0 mEq/L
		Chloride		106		98-107 mEq/L
		C02		29.0		24.0-34.0 mEq/L
		Glucose		116		80-120 mg/dl,
		Bilirubin, total		0.2		0.2â1.9 mg/dL
	AST ALP Protein				26 53 6.8		5-40 IU/L 30-157 IU/L 6.0-8.4 gidL
	2120				2121		Reference Range
BUN			13			7â24 mg/dL
Creatinine			1.2	1.2		0.5â1.2 mg/dL
Calcium			9.0	8.8		8.5â10.5 mg/dL
Albumin			4.2			3.5-5.0 g/dL
ALT			23	NT		5â40 IU/L
Amylase			738	265		10-110 IU/L
Lipase			3970*	320		31-186 IU/L
			335	NT		13-86 IU/L
Cholesterol			NT	165		Recommended (desirable): < 200 mg/dL
Triglyceride			NT	155		Recommended (desirable): < 250 mg/dL

2120

2121

Reference Range

WBC

RBC

ESR

12.1

4.93

10.8

4.76

5-10 x 10⁹/1.

4.0-5.0 x

0â20 mm/h

HDL 35 Recommended (desirable): 60 mg/dL LDL NT 99 Recommended (desirable): 130 mg/dL *Lipemic sample, results rechecked.

Abbreviations: ALT, alanine aminotransferase; ]GT, gamma glutamyltransferase; HDL, high-density lipoprotein; LDL, low-density lipoprotein; NT, not tested.

Table 2-9 " HEMATOLOGY

Abbreviations.' ESR, erythrocyte sedimentation rate; NT, not tested.

You are studying synaptic transmission and discover a treatment that reduces the proportion of failures at your synapse (the fraction of presynaptic action potentials that do not produce a postsynaptic response).

a.) Propose mechanisms that might explain what is changed by this treatment. Include discussion of interpretations from classical Quantal Analysis as well as more recent work on Silent Synapses.

b.) Propose one or more experiments that could test what is actually changed at your synapse by this treatment. Include a brief description of possible results and how you would interpret the results.

indigozebra457

Experiment 2: Analysis of phosphate acquisition mutants of E. coli.

Present the table from the handout on which you recorded your results. (No marks, will be used as a guide for marking Q2) â¨

Strain	Growth on Pi plates(yes/no)	Growth on G3P plates (yes/no)	AP-ase assay colour
1	No	Yes	Yellow
2	No	Yes	None
3	Yes	Yes	Yellow
4	Yes	Yes	None

Use your results to determine the genotype of each strain (bacteria are haploid, so you would use the terms â(loss of function) mutantâ or âwild typeâ) and complete the table below. (1 mark) â¨

	Genotype
Strain	Alkaline phosphatase	Pi transporter
1
2
3
4

Explain how you reached your conclusions for the genotype of each strain. (1 mark) â¨

Why is growth on G3P is an important control for this experiment? (2 marks) â¨

Suppose the DNA sequences for the alkaline phosphatase and the Pi transporter genes from â¨strain 1 were determined. If both genes are found to contain a single base change compared to the wild type sequences, what could you conclude about the effect of each mutation on the encoded protein? (2 marks) â¨