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chocolatefish240Lv1

28 Sep 2019

I have some questions about microscopy that I do not fullyunderstand:

Single particle tracking (SPT)

Fluorescence recovery after photobleaching (FRAP)

Stimulated emission depletion (STED)

1. Are those each a microscope or is it atool that are used by microscopes?

2. There are different fluorescence microscopes; wide-field,confocal and Two-photon microscopes.

DO we have only ONE fluorescence microscope which by changingthe light and filter makes it to widefield microscopy? Or are thosenames (wide-field, confocal and Two-photon microscopes) eachdescribing an individual microscopy?

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