Question

What volumes can you obtain on the large volume pipette? Thesmall volume pipette?(5pts)

How many wells on your ELISA plate do you use for the wholeexperiment?(4pts)

Once you have loaded your ELISA plate how long do you incubateand at what temperature? (2pts)

During your wash steps what solution did you use?(2pts)

How many times did you wash?(2pts)

What antibody did you use in this ELISA (be complete)? (4pts

What color indicates a positive result? (2pts)

What did your results indicate about your patients? Include atable or chart depicting results. (4pts)

Did you yield the correct results? Attach a picture of yourresults and the results you should have obtained if they differ.(4pts)

Please answer all- Thank you!