Compare and contrast your results for Salmonella typhimurium, Shigella boydii, and Escherichia coli for the MAC plate, HE plate, ODC plate, KIA slant, and motility nitrate deep. How could these tests be used in a diagnostic setting?
How does this compare to your results in the KIA slant with MR-VP broth?
Compare and contrast your results for Salmonella typhimurium, Shigella boydii, and Escherichia coli for the MAC plate, HE plate, ODC plate, KIA slant, and motility nitrate deep. How could these tests be used in a diagnostic setting?
How does this compare to your results in the KIA slant with MR-VP broth?
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IMVic (Indole, Methyl Red, Voges-Prokauer, and Cietrate) Reactions
Name___________________________________________________________ Date _________________________
Record your results after incubation in the table.
SIM | MRVP | Citrate | |||||
Organism | Sulfide (+ or -) | Indole (+ or -) | Motility (+ or -) | MR (+ or -) | VP (+ or -) | Slant Color | Citrate (+ or -) |
Salmonella typhimurium | NA | NA | NA | NA | |||
Escherichia coli | |||||||
Enterobacter aerogenes | NA | NA | NA | ||||
Klebsiella pneumonia | NA | NA | NA | NA |
Interpretation and Questions:
The IMViC tests are based on some enzymatic/physiological process. Name the substrates for the enzymes in the indole, MRVP reactions, and citrate test.
Is it possible for an organism to be MR+ and VP+? Why or why not?
Why is it important to inoculate only a single tube per organism when performing the MRVP test?
During lab, your lab partner bumps into your, jostling your arm while you are inoculating your SIM tube. Is it still possible to reliably evaluate the sulfide, indole, and motility results from this tube? Which reactions will still be accurate?
The case study describes a cluster of food poisoning incidences. If the causative agent is Salmonella spp., then give your prediction of the IMViC test results.