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Determine the outcome for the lac operon genotype shown below:

I+P-OcZ+Y-/I-P+O+Z+Y-

Assume lactose is present.

Select one:

Functional beta-galactosidase and functional permease are produced.

Only functional beta-galactosidase is produced. Functional permease is NOT produced.

Only functional permease is produced. Functional beta-galactosidase is NOT produced.

Functional beta-galactosidase is NOT produced. Functional permease is NOT produced.

Determine the outcome for the lac operon genotype shown below:

I+P-OcZ+Y+/IsP+OcZ+Y+

Assume lactose is present.

Select one:

Functional beta-galactosidase and functional permease are produced.

Only functional beta-galactosidase is produced. Functional permease is NOT produced.

Only functional permease is produced. Functional beta-galactosidase is NOT produced.

Functional beta-galactosidase is NOT produced. Functional permease is NOT produced.

etermine the outcome for the lac operon genotype shown below:

I+P+OcZ-Y+/IsP+O+Z+Y+

Assume lactose is absent.

Select one:

Functional beta-galactosidase and functional permease are produced.

Only functional beta-galactosidase is produced. Functional permease is NOT produced.

Only functional permease is produced. Functional beta-galactosidase is NOT produced.

Functional beta-galactosidase is NOT produced. Functional permease is NOT produced.

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Sixta Kovacek
Sixta KovacekLv2
30 Sep 2019
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Related questions

Determine the outcome for the lac operon genotype shown below:

1. I+P+OcZ+Y-

Assume lactose is present.

2. I+P+OcZ+Y-

Assume lactose is absent.

3. I+P+OcZ+Y+

Assume lactose is present.

4. IsP+O+Z+Y+

Assume lactose is present.

5. I+P-OcZ+Y-/I-P+O+Z+Y-

Assume lactose is absent.

6. I+P+OcZ-Y+/IsP+O+Z+Y+

Assume lactose is present.

7. I+P+O+Z+Y-/IsP+OcZ+Y-

Assume lactose is present.

For this question, focus on four parts:

I, the gene that produces the repressor protein;

P, the promoter region where RNA polymerase binds to initiate transcription;

O, the Operator site, where the repressor binds. When the repressor binds, it prevents transcription; and

ZYA, the three structural genes, all of which are required for the successful utilization of lactose as a food source.

In the left column of the table below, we indicate the genetic composition at the lac operon of particular strains of E. coli cells. A "+" (plus sign) following the letter indicates the wild-type allele (or normally functioning version of the gene). For I, P, and ZYA, a "-" (negative sign) following the letter indicates the mutant allele (or non-functional version of the gene). For the O allele, a "C" indicates the mutant or constitutive allele. It is not functional in that the repressor cannot bind, but it is functional in that transcription can still occur.

Given the conditions stated in each column, please indicate for each genotype whether lac mRNA is produced and at what level by typing in one of the following words in each cell in the table:

"Abundant"

"Minimal"

"None"

The completely wild-type genotype has already been filled in.

Cellular conditions:
Genotype Glucose present (low cAMP) and no lactose Glucose present (low cAMP) and lactose present No glucose (high cAMP) and lactose present
I+ P+ O+ ZYA+ None Minimal Abundant
I- P+ O+ ZYA+
I+ P- O+ ZYA+
I+ P+ Oc ZYA+
I+ P+ O+ ZYA-

1. The following shows the genotype of a partial diploid bacterial cell - where one chromosomal region containing the lac operon in E,coli is given, and the other fragment is from a plasmid carrying another lac operon from another source. The two are separated by a slash (/).

The possible answers indicate with a ʺ+ʺ or a ʺ-ʺ whether β-galactosidase would be expected to be produced at induced levels under two circumstances: 1) first in the absence of lactose and 2) second in the presence of lactose. (Assume that glucose is not present in the medium.)

Genotype C: Is Oc Z+/ Fʹ I + O+ Z+
KEY:

I+ = wild-type repressor
I- = mutant repressor (unable to bind to the operator)
Is = mutant repressor (insensitive to lactose)
O+ = wild-type operator
Oc = constitutive operator (insensitive to repressor)

a. +,+ d. -,+

b. -,- e. cannot tell

c. +,-

2. The following shows the genotype of a partial diploid bacterial cell - where one chromosomal region containing the lac operon in E,coli is given, and the other fragment is from a plasmid carrying another lac operon from another source. The two are separated by a slash (/).

The possible answers indicate with a ʺ+ʺ or a ʺ-ʺ whether β-galactosidase would be expected to be produced at induced levels under two circumstances: 1) first in the absence of lactose and 2) second in the presence of lactose. (Assume that glucose is not present in the medium.)

Genotype D: I- O+ Z+/ Fʹ I- O+ Z+
KEY:

I+ = wild-type repressor
I- = mutant repressor (unable to bind to the operator)
Is = mutant repressor (insensitive to lactose)
O+ = wild-type operator
Oc = constitutive operator (insensitive to repressor)

a. +,+ d. -,+

b. -,- e. cannot tell

c. +,-

3. The following shows the genotype of a partial diploid bacterial cell - where one chromosomal region containing the lac operon in E,coli is given, and the other fragment is from a plasmid carrying another lac operon from another source. The two are separated by a slash (/).

The possible answers indicate with a ʺ+ʺ or a ʺ-ʺ whether β-galactosidase would be expected to be produced at induced levels under two circumstances: 1) first in the absence of lactose and 2) second in the presence of lactose. (Assume that glucose is not present in the medium.)

Genotype E: I + O+ Z+/ Fʹ I + O+ Z+
KEY:

I+ = wild-type repressor
I- = mutant repressor (unable to bind to the operator)
Is = mutant repressor (insensitive to lactose)
O+ = wild-type operator
Oc = constitutive operator (insensitive to repressor)

a. +,+ d. -,+

b. -,- e. cannot tell

c. +,-

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