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1.) How many DNA fragments will be generated if a linear DNA molecule is cut 3 times?

2.) How many DNA fragments will be generated if a circular DNA molecule is cut 3 times?

3.) What does the size of a particular restriction fragment tell us if a large DNA molecule was cut with the combination of HindIII and EcoR1? The position of the fragment within the DNA molecule? The enzyme that cut in a particular location? The distance between the two cuts?

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Bunny Greenfelder
Bunny GreenfelderLv2
28 Sep 2019

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Related questions

1. A restriction enzyme has a 6 base recognition sequence. How often would it cut DNA in a typical genome?

2. A linear DNA fragment is cut with EcoRI and HindIII:

EcoRI: 1,5,6 kb fragments

BamHI: 3,9 kb fragments

EcoRI and BamHI together: 1,2,4,5 kb fragments

What is the arrangement of the restriction enzyme sites (E=EcoRI, B=BamHI, numbers indicate distance in kb between restriction enzyme sites)?

1.) A restriction recognition site is repeated 1 times on a circular DNA molecule. If you treat this DNA molecule with this restriction enzyme, how many DNA fragments will you get?

a. 1

b. 2

c. 3

d. Restriction enzyme does not cut a circular molecule

2.) In an agarose gel DNA electrophoresis, the DNA fragments are separated based on their

a. Size

b. Electrical charge

c. Nucleotides sequences

d. Density

e. All of the above

3.) DNA molecules are usually separated using agarose gel rather than polyacrylamide because:

a. Agarose polymerizes faster than acrylamide gel

b. Polyacrylamide gel has pore size larger than the size of DNA molecules

c. Agarose gel do not have pores on tis matrix and can hold large DNA molecules

d. Agarose gel has larger pore size similar to the size of DNA molecules

4.) Restriction endonucleases

a. Randomly recognize and cut DNA sequences

b. Hydrolysis the phosphodiester bond of DNA backbone

c. Recognize specific sequence on double stranded DNA

d. A and B

e. B and C

Why do you need to wear gloves and goggles when working with ethidium bromide (EtBr).

What is the purpose of the non-digested plasmid DNA sample?

If a restriction enzyme cuts a circular plasmid two times how many linear fragments will be generated?

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