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I am doing an experiment lab report. Our question proposed is what will be the effect of the combination of earthworms and organic fertilizers on the growth of radish plants. It is expected that the average growth of radish plants that contain worms and organic fertilizer will have the greatest growth compared to radish plants grown with only organic fertilizer, only soil or only worms.

A. How can I put the experiment into a bigger context. (i.e.: Who cares?? Why does it matter?) After I get the results, how can I relate this to the world, and why these results should matter to people. I think it has to do with farming and the enviroment but I'm not sure how to put it into words.

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Deanna Hettinger
Deanna HettingerLv2
28 Sep 2019

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Related questions

Lab Topic 1: The Process of Science

Introduction

1. Which of the following questions would you consider appropriate scientific questions that can be tested using the scientific method? For the statements that can be tested using the scientific method, explain how your experiment would be set up. Explain the different steps in the scientific method as they relate to this statement.

a. Does the pH of the soil affect the color of hydrangea flowers?

b. What properties of the soil influence the color of hydrangea flowers?

c. Do hydrangeas look more beautiful when grown in the right soil?

d. Was The Day After Tomorrow a better movie than Terminator?

e. Does grass require fertilizer in order to grow?

2. Read this hypothesis and answer the questions below:

Plants kept closer to windows grow larger than plants kept several feet away from windows.

a. Indicate the dependent variable:

b. Indicate the independent variable:

c. List 3 other variables that must be kept constant in this experiment (the control variables).

d. Write a prediction based on the above hypothesis.

Exercise 1.1

Take a Deep Breath

Students will need to take a picture of themselves or their group walking up and down on the step or doing other exercise. Include a note with your name and date on an index card in the picture. Insert picture here:

1. Identify the following in the experimental design. You will need to refer to the protocol to answer this question.

a. Independent variable:

b. Dependent variable:

c. Experimental dataset:

d. Control dataset:

e. Hypothesis:

2. Fill in the data for each individual in your group for the Breath experiment.

Length of time breath can be held (seconds)

Individual Tested

Resting

After 1 minutes of exercise

After 5 minutes of exercise

Average (take the average for each column)

3. Look at the averages for all individuals in your group. Was the hypothesis you stated in the previous question supported by the “average data”? Explain your answer!!!

4. Now look at the data for each individual separately. Was there data for anyone in your group that did not support the hypothesis? If yes, explain whythis could be.

5. How many replicates did your group have?________________ Why is it good to have more than one replicate?

Exercise 1.2

Let’s Graph

In this exercise, you will be graphing the data that you collected in exercise 1.1.

1. In exercise 1.1, you identified the independent and dependent variables. Which one goes on the X axis of the graph and which goes on the Y axis?

2. Will you make a line or a bar graph of your data? Explain why you chose this type of graph.

3. You will need to use excel to generate a graph of your data. On this graph, you will need to label the title, independent variable, dependent variable and possibly the legend. You will need to select an appropriate scale so the data is spread out on the graph.

*Consult with your instructor to see how he/she would like for you to submit your graph. You might work on it in class and submit it through D2L or you might submit it at a different time. If you are taking the online version of the class, you will need to use Excel or word to make the graph.

Exercise 1.3

You will need to get the data under exercise 1.3 in the protocol sheet to complete these sections.

1. What is the dependent variable?

2. What is the independent variable?

3. Which, if any, of these individuals has diabetes? ___A or B ___?

4. What data do you have to support your hypothesis?

5. Construct a graph showing the time after eating each group had the greatest blood glucose levels.

Please I need help answering those questions i always lose bunch of points i'm not doing it correctly.

Use information from Nature Reviews Microbiology to answer the following question.What is the role of EF-G and EF4 in protein synthesis? Under what circumstances does EF4 participate in translation and what is its role?

from this reading The Highly Conserved LepA Is a Ribosomal Elongation Factor that Back-Translocates the Ribosome Qin Y,Polacek N, Vesper O, Staub E, Einfeldt E, Wilson D.N, and Nierhaus N.H. Cell 127: 721-733 (2006).

Q2. In which species is Lep A found and in which ones is it absent? Is the result expected based on your knowledge of the endosimbiosis theory?

Q3.

3a What is the percentage of identity between LepA and its ortholog genes?

3b. (5pts) Indicate what domains are conserved between Ecoli EFG and LepA, and which ones are not.

3c. What do grey and black boxes in the sequence alignment indicate?

Q4. Analysis of in vivo experiments shown in Figure 3. Only analyze graph on the left side of the figure.

4a. What is the difference between the bacteria whose growth are shown by the blue and the green curves? What can be concluded about the effect of EFG on bacterial growth?

4b. Which protein is expressed by bacteria whose growth is shown by the yellow curve? Does the expression of this protein affect bacterial growth? Compare the yellow and blue curves. How can this result be explained?

4c. Explain why the addition of IPTG results in lower bacterial growth. Compare the yellow and red curves?

4d. Why is the green curve an important control of the experiment? The green curve.

The next questions are based on the reading of the article Elongation factor 4 (EF4/LepA) accelerates protein synthesis at increased Mg2+ concentrations.

Elongation factor 4 (EF4/LepA) accelerates protein synthesis at increased Mg2+ concentrations Pech M, Karim Z, Yamamoto H, Kitakawa H, Qin Y, and Nierhaus K. PNAS 108: 3199-3203(2011)

Studies done in E. coli using a knockout mutant of the lepA gene (ΔlepA , lepA gene has been deleted) did not show any phenotype when grown in rich LB medium. This result was unexpected due to the high level of conservation of EF-4 among species.

Q5. Equal amounts of wild type and mutant cells (lacking the lepA gene) were mixed and co-cultured for 24h. The population distribution was determined every 4 hours. Figure 1 indicates changes in the % of mutant cells over several generations.

Q5a. What is the question that researchers addressed in the experiments shown in Figure 1?

Q5b. Which curve represents the control of the experiment? Do the two strains grow at similar rate? How do you know?

Q5c. Do high salt concentration and/or low temperature have any effect on the growth of mutant cells?

Q5d. What can you conclude about the role of LepA on bacteria growth? When is this protein important?

Q6.

6a. (6pts) Where in the cell is EF4 located? Did you expect that this would be the EF4 location? Why or why not?

What is the question that the researchers want to address in the experiment shown in Figure 2?

6c. Analyze the green and red bars graph shown in Figure 2. What technique was used to detect EF4? Is there any change in EF4 localization?

Q7. Summarize the main points of the model shown in Figure 4

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