What electron or light microscopy technique would you use toobserve the following?
a) the fine detail of the outer surface of hair cells in theinner ear.
b) the interior and exterior of surfaces of the plasma membraneto support the Fluid-Mosaic Model of membrane structure.
c) very thin cross sections made in Epon in order to visualizethe fine structure of cellular organelles and cell architecture inthe cell interior.
d) particulate matter (like large multisubunit enzyme complexesor viruses) so as to exhibit the subunit structure. Negativestaining.
e) specifically localize a molecule like tubulin in a cell usinga light microscope.
f) the highly organized structure at the molecular level of thespindle of a dividing cell using the lightmicroscope.
g) technique using lasers to optically section large specimens;the images may be computer-enhanced.
h) living cells without killing them by adding stain.
i) a three-dimensional view (almost bas-relief view)of chromosomes in a dividing cell.
j) living cells with high resolution and with extraordinarycontrast at extremely low light levels to keep the cells aliveduring the observation.
k) viewing poorly stained specimens by focusing light on thespecimen alone; the specimen stands out brightly against a darkerbackground
What electron or light microscopy technique would you use toobserve the following?
a) the fine detail of the outer surface of hair cells in theinner ear.
b) the interior and exterior of surfaces of the plasma membraneto support the Fluid-Mosaic Model of membrane structure.
c) very thin cross sections made in Epon in order to visualizethe fine structure of cellular organelles and cell architecture inthe cell interior.
d) particulate matter (like large multisubunit enzyme complexesor viruses) so as to exhibit the subunit structure. Negativestaining.
e) specifically localize a molecule like tubulin in a cell usinga light microscope.
f) the highly organized structure at the molecular level of thespindle of a dividing cell using the lightmicroscope.
g) technique using lasers to optically section large specimens;the images may be computer-enhanced.
h) living cells without killing them by adding stain.
i) a three-dimensional view (almost bas-relief view)of chromosomes in a dividing cell.
j) living cells with high resolution and with extraordinarycontrast at extremely low light levels to keep the cells aliveduring the observation.
k) viewing poorly stained specimens by focusing light on thespecimen alone; the specimen stands out brightly against a darkerbackground
