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28 Sep 2019
This DNA sequence(5'-TACAAAAGAAGAACAGACTAATCCACGCAATTTGTAGTGGTAGTGGTGGTG-3') wasinserted into a plasmid that was inserted into E coli whichsubsequently grew into a protein XYZ in high yield. With theunpurified cell culture how would you purify the protein XYZ fromthe mixture and confirm with at least two different methods theindentity of XYZ?
This DNA sequence(5'-TACAAAAGAAGAACAGACTAATCCACGCAATTTGTAGTGGTAGTGGTGGTG-3') wasinserted into a plasmid that was inserted into E coli whichsubsequently grew into a protein XYZ in high yield. With theunpurified cell culture how would you purify the protein XYZ fromthe mixture and confirm with at least two different methods theindentity of XYZ?
Nelly StrackeLv2
28 Sep 2019