You have isolated a new nuclear protein, whose expression level is signficantly increased in cancer. Careful sequence analysis reveals the following sequence in the N-terminus of this protein (the positions of the amino acids are indicated): Pro(1)-Pro(2)-Val (3)-Ala(4)-Arg(5)-Lys(6)-Lys(7)-Arg(8)-Lys(9)-Lys(10)-Lys(11)-Tyr(12)-Ile(13)â¨. A construct in which amino acids 1-10 are deleted does not localize to the nucleus. However, when you fuse amino acids 1-10 to GFP, GFP remains in the cytosol.
Which of the following statements best describes the conclusion that you would draw from this experiment?
a)amino acids 1-10 are necessary but not sufficient to direct the protein's import into the nucleus
b)amino acids 1-10 are necessary and sufficient to target the protein into the nucleus
c)deletion of amino acids 1-10 disrupt the import of all proteins into the nucleus
d)amino acids 1-10 are sufficient for the nuclear import of this protein
e)amino acids 1-10 are critical for nuclear import because they interact with the Ran GTPase
You have isolated a new nuclear protein, whose expression level is signficantly increased in cancer. Careful sequence analysis reveals the following sequence in the N-terminus of this protein (the positions of the amino acids are indicated): Pro(1)-Pro(2)-Val (3)-Ala(4)-Arg(5)-Lys(6)-Lys(7)-Arg(8)-Lys(9)-Lys(10)-Lys(11)-Tyr(12)-Ile(13)â¨. A construct in which amino acids 1-10 are deleted does not localize to the nucleus. However, when you fuse amino acids 1-10 to GFP, GFP remains in the cytosol.
Which of the following statements best describes the conclusion that you would draw from this experiment?
a)amino acids 1-10 are necessary but not sufficient to direct the protein's import into the nucleus
b)amino acids 1-10 are necessary and sufficient to target the protein into the nucleus
c)deletion of amino acids 1-10 disrupt the import of all proteins into the nucleus
d)amino acids 1-10 are sufficient for the nuclear import of this protein
e)amino acids 1-10 are critical for nuclear import because they interact with the Ran GTPase
Related textbook solutions
Related questions
For the following strand of DNA, use the table below to provide: bases for the complementary mRNA and the associated amino acids. What do you notice about the genetic code for proline in the peptide? How would the loss of the first G or switching that G to a T affect the structure and function of this protein?
DNA: GGC CTA CAA GCC GGA ACA GTT ACC GCG TAA CGA
RNA: ??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ???
Amino Acid:??? ??? ??? ??? ??? ??? ??? ??? ??? ??? ???
(3 Letters)
Genetic Code Table (mRNA)
| 1st position (5' end) | 2nd position (middle) | 3rd position (3' end) | |||
| U | C | A | G | ||
| U | Phe F Phe F Leu L Leu L | Ser S Ser S Ser S Ser S | Tyr Y Tyr Y STOP STOP | Cys C Cys C STOP Trp W | U C A G |
| C | Leu L Leu L Leu L Leu L | Pro P Pro P Pro P Pro P | His H His H Gln Q Gln Q | Arg R Arg R Arg R Arg R | U C A G |
| A | Ile I Ile I Ile I Met M | Thr T Thr T Thr T Thr T | Asn N Asn N Lys K Lys K | Ser S Ser S Arg R Arg R | U C A G |
| G | Val V Val V Val V Val V | Ala A Ala A Ala A Ala A | Asp D Asp D Glu E Glu E | Gly G Gly G Gly G Gly G | U C A G |
