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28 Sep 2019
An ampicillin-resistant, tetracycline-resistant plasmid iscleaved with EcoRI, which cuts within the tetracycline gene. Thecut plasmid is ligated with EcoRI-digested Drosophila DNA toprepare a genomic library. This DNA is then used to transform E.coli K12. A) Under what conditions should you grow bacteria inorder to select for transformed cells (those that have taken up aplasmid)? B) How can you identify bacteria carrying a plasmid thatcontains a fragment of the Drosophila DNA? C) How can you explainthe presence of colonies that are resistant to bothantibiotics?
An ampicillin-resistant, tetracycline-resistant plasmid iscleaved with EcoRI, which cuts within the tetracycline gene. Thecut plasmid is ligated with EcoRI-digested Drosophila DNA toprepare a genomic library. This DNA is then used to transform E.coli K12. A) Under what conditions should you grow bacteria inorder to select for transformed cells (those that have taken up aplasmid)? B) How can you identify bacteria carrying a plasmid thatcontains a fragment of the Drosophila DNA? C) How can you explainthe presence of colonies that are resistant to bothantibiotics?
Nestor RutherfordLv2
28 Sep 2019
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