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Bacteriophages are used as vectors in genetic engineering to insert new genes into bacteria. Describe the process that makes this genetic recombination possible.

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Beverley Smith
Beverley SmithLv2
28 Sep 2019
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Related questions

Q1.

You are a genetic engineering hired by the biotechnology company, Genes 'R' Us, to use genetic engineering to develop a new rose that glows. Place the following steps in the order you would need to perform them to successfully genetically engineer the rose with this trait.

-Extract all of the DNA from a firefly cell

-Use traditional plant breeding techniques to improve the performance of the rose, which has the new glowing gene (transgene)

-Clone the single gene that encodes the glowing protein

-Insert the glowing gene into single rose plant cell using either the gene gun or agrobacterium

-Modify the glowing gene so it acts In the way you want it once its inside the rose plants

-Identify the firefly as an organism that would have a gene encoding a protein for a ‘glowing trait’

Q2.

Genetic engineering is different from traditional plant breeding in that (select all that apply)

___genetic engineering 'physically' removes DNA (genes) from one organism and inserts them into another

___plant breeding and classical biotechnology relies on natural sexual reproduction to create variation while genetic engineering introduces variation one or a few genes at a time into a single cell.

___genetic variation used in classical biotechnology (plant breeding) comes from mutations in genes already found in that crop species. In genetic engineering, new genes are added to the genetic makeup of a plant

___genetic engineers can move DNA from any organism into any organism, even if they are not in the same species.

Q3.

Which method of plant transformation works and depends upon an organism that is a "naturally occurring" genetic engineer?

A)Gene Gun

B)Agrobacterium

C)Microneedle insertion into a plant egg cell

D)Electric Shock of Plant Cells

Bacteria Traits

Why is it possible for bacterial cells to obtain new genetic traits while humans cannot? Would it ever be possible for human cells to undergo conjugation? Why or why not?

Given what you know about genetic recombination in bacteria, do you think it is possible for the medical community to stay ahead of antibiotic resistance? Why or why not?

How do humans contribute to the development of resistance to antimicrobial drugs?

Three pathways for genetic recombination in bacteria uses the protein RecA. However, in each case, a different set of steps precedes the action of RecA, in catalyzing strand invasion. One of these pathways utilizes an enzyme complex called RecBCD, which binds to double-strand breaks in DNA and exhibit both helicase and single strand nuclease activity. When bacterial cells are co-infected with 2 different strains of bacteriophage l, genes located near certain regions of phage DNA, called CHI sites, recombine at much higher frequencies than other genes do. However, in mutant bacteria lacking RecBCD, genes located near CHI sites recombine at the same frequencies as the other genes. Briefly describe a model of recombination with the above information.

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