1. When subjected to the Gram stain, mycobacteria either stain weakly red or donât stain at all. Explain why, describing the differences between the cell envelopes of acid-fast, gram positive, and gram negative bacteria.
2. Synthesizing cell walls with mycolic acids requires a great deal of energy. Do you expect acid-fast bacteria to grow fast or slow compared to other bacteria? Explain.
3. What are the chemical differences between the Gram stain and the acid fast stain?
4. Describe how the LIVE/DEAD stain distinguishes between living and dead cells.
5. What results would you expect from spores? Explain your answer.
6. How can this stain be used to get an accurate measure of living vs. dead cells?
7. Develop a hypothesis that can be tested using the LIVE/DEAD stain
1. When subjected to the Gram stain, mycobacteria either stain weakly red or donât stain at all. Explain why, describing the differences between the cell envelopes of acid-fast, gram positive, and gram negative bacteria.
2. Synthesizing cell walls with mycolic acids requires a great deal of energy. Do you expect acid-fast bacteria to grow fast or slow compared to other bacteria? Explain.
3. What are the chemical differences between the Gram stain and the acid fast stain?
4. Describe how the LIVE/DEAD stain distinguishes between living and dead cells.
5. What results would you expect from spores? Explain your answer.
6. How can this stain be used to get an accurate measure of living vs. dead cells?
7. Develop a hypothesis that can be tested using the LIVE/DEAD stain
