For each of the following, show all math.
1. You wish to have sufficient TSA to make 10 pour plates. Each plate holds approximately 20 ml of agar. The instructions on the container are that 40 grams are to be used to make a liter. How much dehydrated medium do you need?
2. You wish to make 400 ml TSA with a final concentration of 2% NaCl so that it mimics seawater. TSA contains 0.5% NaCl. How much NaCl do you have to add?
3. Your experimental protocol requires that you make a medium with the following composition. How much of each component would you use if you were making 500 ml? § Tryptone 0.25% § Yeast extract 0.05% § NaCl 2% § NaH2PO4 0.06% § MgSO4 0.5% § FeSO4(7H2O) 0.002% § CaCl2 0.01%
4. How does an enriched medium compare to an enrichment medium?
5. Would you expect cellulose to be produced on an agar surface? Explain.
6. Design an experiment to test the UV protection provided by the cellulose pellicle
For each of the following, show all math.
1. You wish to have sufficient TSA to make 10 pour plates. Each plate holds approximately 20 ml of agar. The instructions on the container are that 40 grams are to be used to make a liter. How much dehydrated medium do you need?
2. You wish to make 400 ml TSA with a final concentration of 2% NaCl so that it mimics seawater. TSA contains 0.5% NaCl. How much NaCl do you have to add?
3. Your experimental protocol requires that you make a medium with the following composition. How much of each component would you use if you were making 500 ml? § Tryptone 0.25% § Yeast extract 0.05% § NaCl 2% § NaH2PO4 0.06% § MgSO4 0.5% § FeSO4(7H2O) 0.002% § CaCl2 0.01%
4. How does an enriched medium compare to an enrichment medium?
5. Would you expect cellulose to be produced on an agar surface? Explain.
6. Design an experiment to test the UV protection provided by the cellulose pellicle
