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1) Is it possible to use a 12% separating gel to run a set of proteins that range in MW from 10,000 to 250,000 Da? Explain.

2) You have an enzyme UTSase that is composed of three subunits. Two of the subunits are 34 kD and the other is 17 kD. How many bands will you see on an SDS-PAGE? Explain your answer.

3) What is the purpose of tracking dye in SDS-PAGE? What are the ingredients of sample buffer? Describe functions of each constituent.

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Irving Heathcote
Irving HeathcoteLv2
28 Sep 2019

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Related questions

1) Is it possible to use a 12% separating gel to run a set of proteins that range in MW from 10,000 to 250,000 Da? Explain in detail.

2) You have an enzyme UTSase that is composed of three subunits. Two of the subunits are 34 kD and the other is 17 kD. How many bands will you see on an SDS-PAGE? Explain your answer.

3) In paper electrophoresis, you separated amino acids based on charge. What is the variable this time? How do you know that there is only one variable in this separation?

1) You have separated two proteins, protein A that has migrated 3.5 cm and protein B migrated to a 4 cm distance from the loading point in the SDS-PAGE experiment. The tracking dye migrated to a distance of 6.5 cm. What will be the Rf value of protein B?

2) In paper electrophoresis, you separated amino acids based on charge. What is the variable this time? How do you know that there is only one variable in this separation?

3) You have an enzyme UTSase that is composed of three subunits. Two of the subunits are 34 kD and the other is 17 kD. How many bands will you see on an SDS-PAGE? Explain your answer.

An antibody is compsed of two "heavy" chains (50 kD) and two "light" chains(25 kD), these chains are connected to one another by disulfide bridges. If a sample of antibodies was subject to reduceing SDS-PAGE, how many bands would you expect to see on the resulting gel? Explain your answer. Now a similar question was asked and the given answer was 5 bands, however I do not understand how the disulfide bridges come into play in determining the number of bands produced.

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