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If we consider any typical medium used in biology to grow bacteria and it's classification.. rich, minimal, complex, selective, or differential.

In selective, what characteristic/type of bacteria are the selective for?

In differential, what characteristic/type of bacteria do they differentiate for?

What phenotypes can you see on the differential media and what do those phenotypes indicate?

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Trinidad Tremblay
Trinidad TremblayLv2
29 Sep 2019

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Related questions

A. Indicate whether the following descriptions apply to nutrient, defined, selective,

differential, or enrichment media. If the statement applies to more than one, indicate which ones.

Answers may be used more than once.

____________________ if you want to take an environmental sample and grow a wide variety of

bacteria, you would use this medium

____________________ blood agar is an example, because you can see whether or not microbes

produce substances that lyse red blood cells

____________________ you might make this medium with a specially-formulated salt mixture,

some vitamins, and a refined protein source like peptone

____________________ the lauryl tryptose broth that we used for the presumptive coliform test

is this type of medium- we used it to increase the chance of actually seeing the coliforms

B. Indicate whether the following procedures are best performed with liquid or solid media. If

the statement applies to both, say “both.”

_______________________ you can count colonies of microbes grown with this medium

_______________________ you can grow facultative anaerobes with this medium

_______________________ you can use a spectrophotometer to measure growth in this medium

_______________________ you can prepare this medium by autoclaving it prior to use

Pre-Laboratory Questions

1. Why do bacterial cultures need to be incubated, usually for at least 24 hours, before we can see evidence of bacterial growth that can be observed with the unaided eye?

2. In what ways are the media used in this investigation selective?

3. In what ways are the media used in this investigation differential?

4. Consider a mixture of bacterial species from the same genus, such as Staphylococcus. Some members of this genus are harmful pathogens, while others are not. Would a selective medium alone be a good tool to separate the harmful species from the non-harmful species? Explain your answer.

Post-Lab Questions

1. Would simply adding a pH indicator like phenol red to a general-purpose medium, such as nutrient agar, result in a medium that is selective, differential, both, or neither? Why or why not?

2. Why were you instructed to inoculate each plate with three separate streaks, one of each bacterial species, instead of combining all three species and streaking them all onto the plate together?

3. Blood agar and chocolate agar are commonly-used media in many clinical and research laboratories. Why do you think they were not included in your kit?

4. Were there any discrepancies between what you saw in your experiment and what you expected to see based on the lab manual and your readings in this section?

BACTERIAL GROWTH AND CONTROLLING BACTERIALGROWTH

1. Based upon your results, is your bacteria sample a strictaerobe, strict anaerobe, facultative anaerobe, or microaerophile?Why? – Look at your results from OF Glucose and OF Glucose with oiltest.

2. Did the bacteria grow in the tryptone or malonate media? Whyor why not? – Remember, you can tell if they grew if culture wasturbid (cloudy).

3. For the media used in this portion of the experiment, are themedia complex or synthetic? Are the media selective, differential,both, or neither? Identify the carbon and nitrogen source for eachmedium. – Read more about what these terms mean starting on page178 in your book right below Figure 6.11. Look up each media withthe M? button. Remember, nutrient broth is a complex media, mineralsalts is a defined media.

4. How many possible bacteria were remaining after conductingthese biochemical tests?

5. Using the information in the Identification matrix, whatadditional tests might help you to identify the bacteria in yourcase study? –Look at the View Identification Matrix under the Viewmenu. What tests would you perform? Look for ones that have a mixof + and – because they would help distinguish betweenorganisms.

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