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How do DNA and RNA FISH (fluorescene in situ hybridization) method differ, both in terms of the method and in terms of what they reveal about cells and organisms?

What kinds of questions can DNA FISH answer?

What kinds of questions can RNA FISH answer?

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Patrina Schowalter
Patrina SchowalterLv2
29 Sep 2019

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Related questions

Hello could you help me with the following questions.

1. Which strategy for testing gene function requires that you know more about the gene or pathway you are studying before you start?

a. Forward genetics

b. Reverse genetics

2. Can the Cre-loxP system be used to "knock out" a gene in a wildtype (not genetically modified) mouse?

a. Yes, the Cre recombinase can use any loxP sites, even those that have not been engineered into the genome.

b. No, the Cre-loxP system requires loxP sites to be inserted into the genome by researchers.

3. What is the mechanistic reason that in situ hybridization images are purple?

a. Binding of RNA to a probe results in a purple precipitate.

b. An antibody conjugated to alkaline phosphatase cleaves NBT/BCIP into a purple product.

c. NBT/BCIP in solution is purple.

d. Digoxigenin-labeled nucleotides in the probe react with NBT/BCIP to form a purple product.

4. In situ hybridization detects:

a. RNA

b. Both RNA and protein

c. protein

d. DNA

5. Which is NOT a mechanism for control of mRNA expression by cytoplasmic localization?

a. Localized protection

b. Nuclear export and splicing

c. Active transport along the cytoskeleton

d. Diffusion and local trapping or anchoring

What kinds of methods could be used to study the RNA of cancer cells, specifically of tumors (other than using the RNA binding protein RBM10 as the means of splicing)? Do other methods exist that researchers could use to uncover research about cancer cells in general? Please detail and give examples.

CHAPTER 7 DNA & GENES

Compare and contrast the following biological terms. Think about how they are related and how they differ. Once you have read the chapter add an example of each term or use them in a sentence.

DNA/chromosomes

transcription/translation

DNA polymerase/RNA polymerase

codons/anticodons

operons/ transcription tactors

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