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6 Jan 2019

Is it possible to use RFLP to genotype individuals with FAP Familial Adenomatous Polyposis

? What must the mutation sit in the middle of to do the RFLP experiment?

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Tod Thiel
Tod ThielLv2
8 Jan 2019

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Related questions

Southern blot analysis of the three genotypes found at the β-globin locus revealed a distinctive pattern of RFLP variation among the genotypes, while northern blotting reveals no difference. What results would you expect from native electrophoresis of the protein from the three β-globin genotypes?

(a) A western blot analysis performed on protein from individuals with the three β-globin genotypes shows that βAβA individuals have a single protein band, whereas βSβS individuals have no protein. βAβS heterozygotes have half as much protein as a homozygous individual.

(b) A western blot analysis performed on protein from individuals with the three β-globin genotypes detects a single protein band, so all genotypes look identical by western blotting.

(c) When a western blot analysis is performed on protein from individuals with the three β-globin genotypes, βAβA and βSβS individuals each have one distinct band, whereas βAβS individuals have both bands.

(d) A western blot analysis performed on protein from individuals with the three β-globin genotypes looks very similar to the Southern blot results. βAβA individuals have two bands, βSβS individuals have one band, and βAβS individuals have all three bands.

Answer is C because western blotting uses proteins.

Please explain the northern, southern, and western blots and why a, b, and d were incorrect. Use drawings when necessary.

Southern blot analysis of the three genotypes found at the β-globin locus revealed a distinctive pattern of RFLP variation among the genotypes, while northern blotting reveals no difference. What results would you expect from native electrophoresis of the protein from the three β-globin genotypes? (a) A western blot analysis performed on protein from individuals with the three β-globin genotypes shows that βAβA individuals have a single protein band, whereas βSβS individuals have no protein. βAβS heterozygotes have half as much protein as a homozygous individual. (b) A western blot analysis performed on protein from individuals with the three β-globin genotypes detects a single protein band, so all genotypes look identical by western blotting. (c) When a western blot analysis is performed on protein from individuals with the three β-globin genotypes, βAβA and βSβS individuals each have one distinct band, whereas βAβS individuals have both bands. (d) A western blot analysis performed on protein from individuals with the three β-globin genotypes looks very similar to the Southern blot results. βAβA individuals have two bands, βSβS individuals have one band, and βAβS individuals have all three bands. Answer is C because western blotting uses proteins. Please explain the northern, southern, and western blots and why a, b, and d were incorrect. Use drawings when necessary.

Consider two genes in a large, randomly mating population of turtles with no movement of individuals in and out of the population and no mutation. The two alleles at one gene, L and M, do not affect fitness. The two alleles at the other gene, T and t, do affect fitness -- they affect the thickness of turtle shells, and the degree to which they are protected from predation. TT individuals have thick shells, which repel predators, and survive best. Tt individuals have medium shell thickness and survive 88% as well as do TT individuals. tt individuals have thin shells and are easy for predators to eat; they survive only 14% as well as do TT individuals.

a) In a population of ADULT turtles, the frequency of individuals with the MM genotype is 0.06. (Calculate the frequency of individuals with the LM genotype. )

b) In a population of gametes at the start of a generation, the frequency of the t allele is 0.23. (Calculate the frequency of zygotes with the Tt genotype.)

c) Continuing from part (b), calculate the frequency of the adults that survive from the zygotes that have the Tt genotype.

d) Continuing from part (c), calculate the frequency of the t allele in the gametes that will produce the next generation.

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