Question

I would like to conduct a simple dimerization experiment forsome protein I'm collecting from a cultured cells. My thought is,that if I'm running a non-reducing, denaturing PAGE gel, thenremoving beta-mercaptoethanol/DTT from the sample buffer should beenough to allow di-sulfide bonds to form.

I have seen several authors incubate the cells first with a drugcalled BSS that diffuses across membranes and creates proteincross-links. I may be wrong, but the use of this drug seems moreappropriate when trying to follow up with an immunoprecipitation ora pull-down assay.

If anyone has any experience with this, could you pleaseenlighten me?