3
answers
0
watching
344
views
2 Jun 2018
5. On a given microscope, the numerical apertures of thecondenser and low power objective lenses are 1.25 and 0.25,respectively. You are supplied with a filter that selects awavelength of 520nm.
a.) What is the limit of resolution on this microscope? Pleasebe specific to how you came up with this answer, what formula didyou use? b.) Will you be able to distinguish 2 points that are300nm apart as being separate, or will they blur into one?
6. on the same microscope as in Question#5, the high dryobjective lens has a numerical aperture of 0.85, what is the limitof resolution on this microscope, and will you be able todistinguish 2 points that are 300 nm apart as being separate, orwill they blur into one? Explain answer.
5. On a given microscope, the numerical apertures of thecondenser and low power objective lenses are 1.25 and 0.25,respectively. You are supplied with a filter that selects awavelength of 520nm.
a.) What is the limit of resolution on this microscope? Pleasebe specific to how you came up with this answer, what formula didyou use? b.) Will you be able to distinguish 2 points that are300nm apart as being separate, or will they blur into one?
6. on the same microscope as in Question#5, the high dryobjective lens has a numerical aperture of 0.85, what is the limitof resolution on this microscope, and will you be able todistinguish 2 points that are 300 nm apart as being separate, orwill they blur into one? Explain answer.
Nestor RutherfordLv2
4 Jun 2018
Already have an account? Log in
