You are to microbiologist for the Minnesota Department of Health. A sample of âsuspectâ ground beef from Jimboâs Grocery in Minneapolis is brought to your lab. After performing a set of serial dilutions on 2 cm3 of the food, you inoculate corresponding agar plates with 0.1 ml of the appropriate diluent. The next day, you count 125 colonies on the nutrient agar plate from the 1 X 10-5 dilution tube. Likewise, you count 206 colonies (109 of which are pink) on a similarly inoculated MacConkey Agar plate from the 1 X 10-4 dilution. You inoculated each plate one-tenth (0.1) ml of sample from the tube. Based on this data, determine the following bacterial population densities (cfu/ml) that were in the original sample of this âburger:
⢠Gram-negative, lactose-fermenting bacilli: ______________ CFU/ml
⢠Non- Gram negative bacteria: _________________ CFU/ml
⢠Total bacteria: ____________ CFU/ml
You are to microbiologist for the Minnesota Department of Health. A sample of âsuspectâ ground beef from Jimboâs Grocery in Minneapolis is brought to your lab. After performing a set of serial dilutions on 2 cm3 of the food, you inoculate corresponding agar plates with 0.1 ml of the appropriate diluent. The next day, you count 125 colonies on the nutrient agar plate from the 1 X 10-5 dilution tube. Likewise, you count 206 colonies (109 of which are pink) on a similarly inoculated MacConkey Agar plate from the 1 X 10-4 dilution. You inoculated each plate one-tenth (0.1) ml of sample from the tube. Based on this data, determine the following bacterial population densities (cfu/ml) that were in the original sample of this âburger:
⢠Gram-negative, lactose-fermenting bacilli: ______________ CFU/ml
⢠Non- Gram negative bacteria: _________________ CFU/ml
⢠Total bacteria: ____________ CFU/ml