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27 Feb 2018

a) Would a prototrophic strain of bacteria be able to grow on minimal medium? Yes/No

b) Would an auxotrophic strain of bacteria be able to grow on minimal medium? Yes/No

c) Would an auxotrophic strain of bacteria be able to grow on complete medium? Yes/No

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Tod Thiel
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28 Feb 2018

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Related questions

An auxotrophic strain of Salmonella enterica is able to grow on rich medium (which contains all the amino acids, vitamins, bases and a variety of carbon sources) but not on minimal medium or on minimal medium supplemented with leucine. If the strain also had a block in the methionine synthesis pathway, what could be the possible genotype(s) of this bacterial strain with respect to the met and leu loci?

1.

met+leu– only
2.

met–leu+only
3.

either met+leu– or met–leu+
4.

either met–leu– or met+leu–
5.

either met–leu– or met–leu+

Baker's yeast, Saccharomyces cerevisiae, is a single-celled, diploid fungus (which is, of course, a eukaryote, that is capable of both meiosis and sexual reproduction).

Wild type yeast can normally grow on solid or liquid minimal medium; you isolate three mutant strains which are no longer capable of growing on minimal medium, however, they can grow on medium supplemented with adenine.

All three strains are homozygous for the underlying alleles.

When you cross mutant strain 1 and mutant strain 2, the offspring cannot grow on minimal medium and require adenine supplementation;

when you cross mutant strain 1 and mutant strain 3, the offspring can grow on minimal medium and do not require adenine.

A. What conclusions can you make about the alleles of mutant strains 1, 2, and 3 and their relationships with each other?

B. What phenomenon is occurring in the cross between mutant strains 1 and 3?

After crossing the F1 generation of the cross between mutant strains 1 and 3, you count and determine the phenotypes of 1,000 colonies (here a colony is equivalent to an individual): 563 colonies that can grow on minimal medium; 437 colonies that require adenine supplementation.

C. What genotypes lead to the phenotypes of the F2? What phenomenon is occurring in the F2 generation

Transducing phage can be used to map bacterial genes. Phages are collected from an E. coli donor strain of genotype cys+leu+thr+ and used to transduce a recipient strain of genotype cys-leu-thr-. Initially the treated recipient population is plated on a minimal medium supplemented with leucine and threonine. Many colonies are obtained.

a. What are the possible genotypes of these colonies?

These colonies are then replica plated onto three different media: 1) minimal plus threonine only. 2) minimal plus leucine only, and 3) minimal. What genotypes could, in theory, grow on these three media?

Of the original colonies, 56% are observed to grow on medium 1, 5% on medium 2, and no colonies on medium 3. What are the actual genotypes of the colonies on media 1, 2, and 3?

Draw a map showing the order of the three genes and which of the outer genes is closer to the middle gene.

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