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11 Nov 2019
Please help! I am very lost!
An HEXAPEPTIDE was sequenced using differing enzyme and chemical reagents. The results of these experiments, along with an explanation of how to interpret the results are provided below. Using this information, provide the 1° structure for this HEXAPEPTIDE. (10 points) 2. be sure to provide WORK/ANSWER for Points B-F! Observations from Sequencing Experiments A. The amino acid content in the peptide was: Y, R, M, K, G, D B. Reactions with the peptide and 1-fluoro-2,4-dinitrobenzene yielded DNP-G C. Treatment of the peptide with chymotrypsin yielded two tripeptides. D. Treatment of the peptide with trypsin yielded three dipeptides. E. Treatment of the peptide with carboxypeptidase yielded methionine. F. N-terminal sequencing of one the chymotrypsin-produced tripeptides (from part C) Explanation and Summary of Protein Sequencing Techniques 1. 1-fluoro-2,4-dinitrobenzene (DNFB) is used to identify the N-terminal amino acid of a peptide, and provides the following product: DNP-AA (where AA = the N-terminal amino 2. 3. 4. acid of the peptide). Trypsin is an enzyme that cleaves a peptide at the C-terminal side of R and K. Chymotrypsin is an enzyme that cleaves a peptide at the C-terminal side of F, Y, and W Carboxypeptidase is an enzyme that cleaves off the C-terminal amino acid of any peptide.
Please help! I am very lost!
An HEXAPEPTIDE was sequenced using differing enzyme and chemical reagents. The results of these experiments, along with an explanation of how to interpret the results are provided below. Using this information, provide the 1° structure for this HEXAPEPTIDE. (10 points) 2. be sure to provide WORK/ANSWER for Points B-F! Observations from Sequencing Experiments A. The amino acid content in the peptide was: Y, R, M, K, G, D B. Reactions with the peptide and 1-fluoro-2,4-dinitrobenzene yielded DNP-G C. Treatment of the peptide with chymotrypsin yielded two tripeptides. D. Treatment of the peptide with trypsin yielded three dipeptides. E. Treatment of the peptide with carboxypeptidase yielded methionine. F. N-terminal sequencing of one the chymotrypsin-produced tripeptides (from part C) Explanation and Summary of Protein Sequencing Techniques 1. 1-fluoro-2,4-dinitrobenzene (DNFB) is used to identify the N-terminal amino acid of a peptide, and provides the following product: DNP-AA (where AA = the N-terminal amino 2. 3. 4. acid of the peptide). Trypsin is an enzyme that cleaves a peptide at the C-terminal side of R and K. Chymotrypsin is an enzyme that cleaves a peptide at the C-terminal side of F, Y, and W Carboxypeptidase is an enzyme that cleaves off the C-terminal amino acid of any peptide.
Lelia LubowitzLv2
19 Apr 2019