If I dissolved 0.124 g of caffeine in 17mL of pyridine, in a cell measured to be 14cm the observed rotation at 20 degree C is -0.53 degree. Calculate the specific rotation of caffeine. The specific rotation of (S)-nicotine at 20 degree C is +72.0 degree. A mixture of (R)-nicotine and its enantiomer are mixed, and this showed an observed rotation of -47 degree. What is the observed rotation of (R)-nicotine at this temperature? What is the %ee of this mixture? What percent of the mixture is (S)-nicotine?