Amino acid side chain charge calculation (approximation)
1. Write out ionic forms of the amino acids and determine acid and base charge forms. 2. Use the Henderson-Hasselbach equation to calculate ratio
3. Assigncharge(approximation):
If 10%, assign plus/minus 0.9 or 0.10 values. If 99% assign whole number (or 1% of whole and 99% of whole). If at pKa assign half value 0.5 (plus/minus)
a) The amino acid sequence of sea lamprey adrenocorticotropic hormone is given below. Given the sequence, identify each ionizing amino acid residue and indicate its charge (-1, 0, or +1) in the completely protonated amino acid form of the polypeptide hormone.
S-V-S-S-P-K-Y-A-M-G-H-F-R-W-G-S-P-D-K-A-T-I
b)
Given the same sequence above, indicate the charge (-1, 0, or +1) on each amino acid residue in
the completely proton dissociated form of the polypeptide hormone.
Amino acid side chain charge calculation (approximation)
1. Write out ionic forms of the amino acids and determine acid and base charge forms. 2. Use the Henderson-Hasselbach equation to calculate ratio
3. Assigncharge(approximation):
If 10%, assign plus/minus 0.9 or 0.10 values. If 99% assign whole number (or 1% of whole and 99% of whole). If at pKa assign half value 0.5 (plus/minus)
a) The amino acid sequence of sea lamprey adrenocorticotropic hormone is given below. Given the sequence, identify each ionizing amino acid residue and indicate its charge (-1, 0, or +1) in the completely protonated amino acid form of the polypeptide hormone.
S-V-S-S-P-K-Y-A-M-G-H-F-R-W-G-S-P-D-K-A-T-I
b)
Given the same sequence above, indicate the charge (-1, 0, or +1) on each amino acid residue in
the completely proton dissociated form of the polypeptide hormone.
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