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26 Nov 2019

A known mixture of compounds A and B were analyzed using HPLCyielding the following results.

Concentration (mg/mL)
A: 1.5
B: 1.1

Peak area
A: 10.9
B: 4.4

Adjusted retention time (tRâ, min)
A: 3.2
B: 6.4

(a) 10.0 mL of solution containing an unknown amount of A werespiked with 12.0 mg of B and subsequently diluted to 25.0 mL. HPLCanalysis of the mixture following the same method yielded areas of6.0 and 6.4 for A and B respectively. What is the concentration ofA in the unknown solution?

I received an answer of 10.1, but I am unsure.If someone could showme how they got to the answer (formula/math), and the answer thatwould be great.

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