Question

2. a) State and describe any three factors that may affect positively or negatively the analytical

utility of molecular fluorescence techniques?

b) Why does the fluorescence excitation spectrum appear to be a mirror image of fluorescence

emission spectrum? Why are they not exact mirror images?

c) What does synchronous fluorescence spectroscopy involve? What kind of spectra is

obtainable using this technique? Are there any advantages of using synchronous

spectrometry in fluorescence techniques?