Question

4.) a.) Given a 25X stock solution of Tris Glycine SDS (i.e. running buffer in gel electrophoresis), what volumes of the stock and DI water should be combined to make 100 mL of a 1X Tris Glycine SDS solution?

b.) You have 1 mL of a previously prepared sample buffer to which you wish to add DTT (which is unstable in solution). What volume of 1M DTT will you need to add to achieve a final concentration of 50 mM DTT in the sample buffer?