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12 Dec 2019
Experiment:
Two buffers starting with solid chemicals. Buffers are chosen: Tris and Phosphate ( KH2PO4). Each buffer is desired as 100ml, .100M at pH 7.5.
grams of Tris needed: 1.211g
grams of KH2PO4 needed: 1.36g
______
Given MOPS Free acid, you are asked to make a .10M buffer at pH 6.8. After dissolving MOPS in water, will have to titrate with HCI or?NAOH? why?
*used one or more of the following equations to answer the question*
Henderson-hasselbalch: pH= pKa + log ((A-)/(HA)
weak acid only: pH = ((pH-log(HA))/2
Weak base only: pH = ((pka +14+ log(A-))/2
Polyprotic intermediate: pH = (pKa1 + pKa2)/2
Experiment:
Two buffers starting with solid chemicals. Buffers are chosen: Tris and Phosphate ( KH2PO4). Each buffer is desired as 100ml, .100M at pH 7.5.
grams of Tris needed: 1.211g
grams of KH2PO4 needed: 1.36g
______
Given MOPS Free acid, you are asked to make a .10M buffer at pH 6.8. After dissolving MOPS in water, will have to titrate with HCI or?NAOH? why?
*used one or more of the following equations to answer the question*
Henderson-hasselbalch: pH= pKa + log ((A-)/(HA)
weak acid only: pH = ((pH-log(HA))/2
Weak base only: pH = ((pka +14+ log(A-))/2
Polyprotic intermediate: pH = (pKa1 + pKa2)/2
Nelly StrackeLv2
13 Dec 2019