2. Read the article âRatiometric Time-Gated Luminescence Probe for Nitric Oxide Based on an Apoferritin-Assembled Lanthanide Complex-Rhodamine Luminescence Resonance Energy Transfer Systemâ⨠by Lu Tian, Zhichao Dai, Xiangli Liu, Bo Song, Zhiqiang Ye, and Jingli Yuan, Anal. Chem. 2015, 87, 10878â10885 (DOI: 10.1021/acs.analchem.5b02347) and answer the following questions.
a) How does the presence of molecular NO lead to a signal for the system described in this article?
b) What advantage does the Tb ion provide in this analysis? (Explain the mechanism for this advantage and the experimental considerations/procedure that are necessary to take advantage of this behavior.)
c) Is this an application of Förster Energy Transfer? If not, how does it differ? If so, what criteria indicate that it is?
d) What function does the PTTA ligand serve?
e) In the inset of figure 2A, the signal appears to deviate from linearity above 600 µM NO. Hypothesize a reason to explain that. Describe an experiment that could be used to test your hypothesis.
2. Read the article âRatiometric Time-Gated Luminescence Probe for Nitric Oxide Based on an Apoferritin-Assembled Lanthanide Complex-Rhodamine Luminescence Resonance Energy Transfer Systemâ⨠by Lu Tian, Zhichao Dai, Xiangli Liu, Bo Song, Zhiqiang Ye, and Jingli Yuan, Anal. Chem. 2015, 87, 10878â10885 (DOI: 10.1021/acs.analchem.5b02347) and answer the following questions.
a) How does the presence of molecular NO lead to a signal for the system described in this article?
b) What advantage does the Tb ion provide in this analysis? (Explain the mechanism for this advantage and the experimental considerations/procedure that are necessary to take advantage of this behavior.)
c) Is this an application of Förster Energy Transfer? If not, how does it differ? If so, what criteria indicate that it is?
d) What function does the PTTA ligand serve?
e) In the inset of figure 2A, the signal appears to deviate from linearity above 600 µM NO. Hypothesize a reason to explain that. Describe an experiment that could be used to test your hypothesis.
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