Question

Prepare 100ml of homogenization buffer. Calculate how to do this with (c1V1=c2V2) where C is concentration and V is volume.

Initial Stock solutions:---------------------- Final Buffer:

50mM TrisCl, pH8.6 ---------------------------10mM TrisCl, pH 8.6

100mM PMSF-----------------------------------1mM PMSF

20mM EDTA--------------------------------------1mM EDTA

20 uM pepstatin A -------------------------------0.2 uM pepstatin A

7.1 M 2-mercaptoethanol ----------------------1 mM 2-mercaptoethanol

Please show your work so I can reason through this! Thanks