7. What are essential amino acids? How are they different from non-essential amino acids? Do all organisms have the same set of essential amino acids? What role do essential amino acids play in human biochemistry?
8. How and why are protein molecules affected by the pH of their environment? How can the Henderson-Hasselbalch equation be used to tell us about the response of protein molecules to changes in pH?
9. Describe the use of affinity chromatography and explain why it is such a powerful technique for purifying proteins. Why is it more powerful than most other methods.
10. Many biological compounds could potentially exist as more than one stereoisomer, but only one steric form is used for a given function. Why is the selection of only one steric form a necessary feature of living organisms? What would happen to the protein product if an organism could randomly add either D- or L-amino acids during protein synthesis.
7. What are essential amino acids? How are they different from non-essential amino acids? Do all organisms have the same set of essential amino acids? What role do essential amino acids play in human biochemistry?
8. How and why are protein molecules affected by the pH of their environment? How can the Henderson-Hasselbalch equation be used to tell us about the response of protein molecules to changes in pH?
9. Describe the use of affinity chromatography and explain why it is such a powerful technique for purifying proteins. Why is it more powerful than most other methods.
10. Many biological compounds could potentially exist as more than one stereoisomer, but only one steric form is used for a given function. Why is the selection of only one steric form a necessary feature of living organisms? What would happen to the protein product if an organism could randomly add either D- or L-amino acids during protein synthesis.
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