(Please explain how to solve)
The restriction enzyme BamHI recognizes the 6 base sequence GGATCC, with the cleavage site between the 2 Gâs, to give G GATCC. The 4 base cutter Sau3A recognizes the sequence GATC and cuts before the first G. This means
a. every Sau3A site is also a BamHI site
b. there are two Sau3A sites for every BamHI site
c. if a BamHI fragment is inserted at a Sau3A site it can be cut back out by BamHI
d. Sau3A fragments can be easily cloned into BamHI sites
(Please explain how to solve)
The restriction enzyme BamHI recognizes the 6 base sequence GGATCC, with the cleavage site between the 2 Gâs, to give G GATCC. The 4 base cutter Sau3A recognizes the sequence GATC and cuts before the first G. This means
a. every Sau3A site is also a BamHI site
b. there are two Sau3A sites for every BamHI site
c. if a BamHI fragment is inserted at a Sau3A site it can be cut back out by BamHI
d. Sau3A fragments can be easily cloned into BamHI sites
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