BIO1022 Study Guide - Final Guide: Open Reading Frame, Tata Box, Start Codon
Document Summary
Rna polymerase binds to the promoter region before the gene. Rna pol(cid:455)(cid:373)e(cid:396)ase (cid:373)o(cid:448)es alo(cid:374)g the te(cid:373)plate st(cid:396)a(cid:374)d f(cid:396)o(cid:373) (cid:1007)" to 5", elo(cid:374)gati(cid:374)g the (cid:373)rna (cid:373)ole(cid:272)ule i(cid:374) the 5" to (cid:1007)" di(cid:396)e(cid:272)tio(cid:374) i(cid:374) (cid:272)o(cid:373)ple(cid:373)e(cid:374)t to the te(cid:373)plate st(cid:396)a(cid:374)d"s nucleotides. The dna strands reform a double helix in the wake of the rna polymerase. The rna transcript of the gene is released, and the rna polymerase detaches. In eukaryotes, pre-mrna is produced, which needs to be modified to form mrna. A 5" (cid:272)ap a(cid:374)d a pol(cid:455)-a tail are added to either end. Untranslated regions (utr) remain between the caps and the actual open reading. Introns are removed via splicing, from spliceosomes frame (orf) The rna directed synthesis of a polypeptide. Small unit of ribosome attaches to mrna. Met-trna binds to the start codon, bringing methionine. Large ribosomal sub-unit joins to form the translation initiation complex- this process requires energy from gtp gdp + pi.
