BIOL 330 Study Guide - Final Guide: National Center For Biotechnology Information, Reading Frame, Polyclonal Antibodies
23 Feb 2017
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Their migration rate depends on their individual size: larger proteins are found at the top (near negative end) because they move slower. Proteins of the same size move through the gel with similar speeds because they bind the same amount of charged gel molecules and therefore have the same amount of negative charge. Proteins run on the gel in two directions, one direction indicates ph and the other indicates size: isoelectric focusing- polypeptide chains are separated in a ph gradient. Takes advantage of the variation in the natural net charges in each protein. Each protein moves to a position in the gradient that corresponds with its isoelectric point. Now proteins will separate according to their size. Larger dna strands and strands bound with proteins move slower = top of gel (negative end) The gel has thousands of copies of dna and there are thousands of copies of the same sequence at each site on the gel.
