BIOC 2580 Study Guide - Midterm Guide: Oxyanion, Isoelectric Focusing, Acid Dissociation Constant
8 Jan 2016
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Peptide bonds formed by condensation- removal of h2o. Basic structure: amino (+ve), carboxylate (-ve), r group. Carbon is the central backbone carbon, - carbon is the (pro, p), tryptophan (trp, w), tyrosine (tyr, y) second Groups that ionize on o or s are neutral when protonated and negative when deprotonated. Groups that ionize on n are positive when protonated and neutral when deprotonated. No group goes from positive to negative when protonated. Calculating the exact ionization state of histidine: side chain has pka=6. 5, at ph 7, major form will be his, but some hish+ is present, ph= pka + log[his]/[hish+, 7=6. 5+log[his]/[hish+, [his]/[hish+]=10^0. 5=3. 2. What percent is deprotonated: = degree of protonation, fraction protonated= 1- , [deprotonated]/[protonated]= /1- =3. 2, =3. 2-3. 2 , (1+3. 2)=3. 2, =0. 76, 76% deprotonated, 24% protonated. Ninhydrin- reacts to give purple colour, yellow for proline: partition chromatography stationary phase- solid particles are chosen with specific properties (ex. silica for h-bonding)
