MBG 2040 Study Guide - Final Guide: Start Codon, Tata Box, Caat Box

• School: University of Guelph
• Department: Molecular Biology and Genetics
• Course: MBG 2040
• Professor: James Uniacke

Document Summary

Robert holley: nucleotide sequence of a yeast rna. Marshal nirenburg and ghobind khorana: the genetic code. Barbara mcclintock: transposable elements, breakage fusion bridge (on corn) Right handed double helix (b-dna) most common. A form dna; right handed, very compact, 12 bp/turn. 0. 34 nm between stacked bases; 3. 4nm per helical turn. Cesium chloride equilibrium-density gradient centrifugation is used to separate 14n and 15n. Replication begins from an origin (oirc in e. coli) An at rich region that undergoes strand separation. Template dna to specify the sequence of the new strand. Substrates: dntps, mg2+, dna polymerase (dnmp)n +dntp (dnmp)n+1 +ppi. 5 dna polymerases in e. coli :1,2,3,4,5. Has 5" to 3" polymerase, and exonuclease activity. 30,000 base nucleotides per minute: one error per 10^8-10^9 nucleotides added. Dana protein binds to the 4, 9bp repeats (ttatncana) Additional dnaa proteins bind cooperatively, forming a complex with oric wrapped aroud. Dnab (helicase) and dnac join, and form a replication bubble.