BIOL472 Study Guide - Midterm Guide: T Helper Cell, Pyruvate Dehydrogenase, Plasmalogen
29 Mar 2016
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Er-golgi network clathrin: from golgi or pm arf (gtpase adaptor protein) Cop1: from golgi (going back to er) arf1. Golgi has kdel receptor for soluble er proteins retrograde transport. Ergic er golgi intermediate compartment: ergic53 transmembrane protein can bind cop1 or copii. Cd4 and 8 t-helper cells, cd20- b cells. Copa alpha subunit of copi, copi coat hetemer. Check to see if mutations cause loss of copa protein. Add purified proteins to permeabilized cells (can use fibroblast line) add sec13,31a, and sar1b . Copii gefs and gtpase (don"t detect proteins) then addsec23a and sec24d (to improve copii coat formation) (detect proteins) then add mutated sample or patient sample (detect proteins) Sec22b (copii cargo proteins) see if copa variants result in aggregation or mislocation. Find area of mutation of copi coat pull down assay perform point mutations on copa should bind di-k motif (used for cargo. Ribophorin-1 negative control (er resident protein) shouldn"t detect.
